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This research article presents the development of multifunctional polymer dots (Pdots) for use in expansion microscopy (ExM), which enhances nanoscale imaging of cellular structures. The Pdots demonstrate significantly higher fluorescence brightness compared to traditional dyes, improving visualization of subcellular components and neuron synapses. The combination of ExM and super-resolution optical fluctuation imaging (SOFI) allows for the resolution of structures down to approximately 30 nm using conventi

Author

Sumit Kumar Pramanik

Language

EN