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Development and Validation of a Method for Determination of Corticosteroids in Pig Fat Using Liquid Chromatography–tandem Mass Spectrometry by Ádám Tölgyesi; Virender K. Sharma; Jenő Fekete is a Chemistry article available to read on EtoBox.

What is Development and Validation of a Method for Determination of Corticosteroids in Pig Fat Using Liquid Chromatography–tandem Mass Spectrometry about?

A new method was developed to determine five corticosteroids (prednisolone, methylprednisone, flumethasone, dexamethasone, and methylprednisolone) in pig fat samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) utilizing an optimized liquid-liquid extraction (LLE) and subsequent solid-phase extraction (SPE) for sample clean-up. In the sample preparation, a pig fat sample was dissolved in n-hexane and then extracted into the methanol-water (50/50, v/v) mixture that enabled extraction of only medium polar corticosteroids and not the non-polar components of matrices. This extract was cleaned-up and concentrated on polymeric Oasis HLB SPE cartridge. Separation involved isocratic solvent (methanol-acetate buffer, pH 5.4) and Ascentis Express Fused-Core type HLPC column; reduced the analysis time to 7.5 min, which is at least two times lower than time required for separation using conventional techniques. Other advantage of the developed method is the minimized ion suppression of LC-MS/MS analysis, which allowed detection of corticosteroids in sub μg/kg. Method was validated according to European Union (EU) Commission Decision 2002/657/EC. Measured parameters such as sele

Who reads Development and Validation of a Method for Determination of Corticosteroids in Pig Fat Using Liquid Chromatography–tandem Mass Spectrometry?

Author

Ádám Tölgyesi; Virender K. Sharma; Jenő Fekete

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)