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Development of a novel liquid chromatography — Evaporative light scattering detection method for bacitracins and applications to quality control of pharmaceuticals by Artemis K. Sarri; Nikolaos C. Megoulas; Michael A. Koupparis is a Chemistry article available to read on EtoBox.

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A novel liquid chromatography method for the direct determination of bacitracin main components (Bc-A, -B1, -B2 and -B3), a basic, cyclic polypeptide antibiotic, was developed and validated, based on ion pairs formation with trifluoroacetic acid (TFA) and evaporative light scattering detection (ELSD). The selected analytical column was the Waters Nova-pak C8 (3.9 x 150 mm), for which the optimum (using modified Simplex algorithm) mobile phase was H2O-ACN (73:27, v/v) containing 0.80 microL mL(-1) of TFA, at a flow rate of 1.0 mL min(-1). Optimized ELSD parameters were: nebulizing gas (nitrogen) pressure=3.5 bar, evaporation temperature=50 degrees C, detector gain=12. Retention time of Bc-B1, -B2, -B3, -A and -F (oxidative degradation product of Bc-A) was 5.3, 5.8, 7.7, 8.7, 15.9 min, respectively, while zinc ions and related peptides were eluted at 1.3-1.9 min. A logarithmic calibration curve was obtained for each component (r>0.998), while the concentration range of total bacitracin was 30-235 microg mL(-1). Detection limits for the individual components were in the range 1.0-1.6 microg mL(-1). The proposed method was applied for the direct determination of Bc components and relat

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Author

Artemis K. Sarri; Nikolaos C. Megoulas; Michael A. Koupparis

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)