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Can I read Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure on EtoBox?
Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure by Feng Qian; Joan A. Aebig; Karine Reiter; Emma Barnafo; Yanling Zhang; Richard L. Shimp Jr.; Kelly M. Rausch; David S. Jones; Daming Zhu; Lynn Lambert; Gregory E.D. Mullen; David L. Narum; Louis H. Miller; Yimin Wu is a Medicine article available to read on EtoBox.
What is Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure about?
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. Published by Elsevier Masson SAS.
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- Author
- Feng Qian; Joan A. Aebig; Karine Reiter; Emma Barnafo; Yanling Zhang; Richard L. Shimp Jr.; Kelly M. Rausch; David S. Jones; Daming Zhu; Lynn Lambert; Gregory E.D. Mullen; David L. Narum; Louis H. Miller; Yimin Wu
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1286-4579)
- Published
- 2009
- Language
- EN
- Field
- Medicine (Life Sciences)