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## Abstract Analysis of posttranslational modifications of proteins is an important issue for understanding the relationship between protein structure and function. Micro‐scale analytical methods capable of elucidating glycan structures are therefore gaining attention in connection with proteomics research. Recent efforts directed toward this goal have successfully distinguished and in some cases identified glycan structures based on collision‐induced dissociation (CID) analysis. Despite these advancements, the identification of isomeric glycans such as high‐__mannose__‐type oligosaccharides, Man~7~GlcNAc~2~, that are closely related structurally, is not yet possible. Using energy‐resolved mass spectrometry (ERMS), we found that these isomers could be distinguished by comparing the intensities of certain fragment ions. ERMS is useful because the data obtained can be treated quantitatively. Furthermore, it was found that discrimination can be easily achieved by analyzing only the energy‐resolved mass spectra of the sodiated isomeric compounds at the stage of MS^__2__^. Thus, the importance and usefulness of ERMS, which provide the factor of activation energy under CID, in analyzing

Author

Ayako Kurimoto; Osamu Kanie

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2007

Language

EN