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The Effect of Tranexamic Acid on Synovium of Patients Undergoing Arthroplasty and Anterior Cruciate Ligament Reconstruction Surgery by Salman Ghaffari; Soroosh Fateh; Fatemeh Faramarzi; Alireza Rafiei; Mehran Razavipour; Parisa Zafari is a scholarly article available to read on EtoBox.

What is The Effect of Tranexamic Acid on Synovium of Patients Undergoing Arthroplasty and Anterior Cruciate Ligament Reconstruction Surgery about?

Preoperative hemorrhage can be reduced using anti-fibrinolytic medicine tranexamic acid (TXA). During surgical procedures, local administration is being used more and more frequently, either as an intra-articular infusion or as a perioperative rinse. Serious harm to adult soft tissues can be detrimental to the individual since they possess a weak ability for regeneration. Synovial tissues and primary fibroblast-like synoviocytes (FLS) isolated from patients were examined using TXA treatment in this investigation. FLS is obtained from rheumatoid arthritis (RA), osteoarthritis (OA), and anterior cruciate ligament (ACL)-ruptured patients. The in vitro effect of TXA on primary FLS was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assays for cell death, annexin V/propidium iodide (PI) staining for apoptotic rate, real-time PCR for p65 and MMP-3 expression, and enzyme-linked immunosorbent assay (ELISA) for IL-6 measurement. MTT assays revealed a significant decrease in cell viability in FLS of all groups of patients following treatment with 0.8-60 mg/ml of TXA within 24 h. There was a significant increase in cell apoptosis after 24 h

Author

Salman Ghaffari; Soroosh Fateh; Fatemeh Faramarzi; Alireza Rafiei; Mehran Razavipour; Parisa Zafari

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN