About this document
Cell Freezing Nad Thawing - Master Cell Bank by Naveen Kumar is a document available to read on EtoBox.
This document provides standard operating procedures for culturing, sub-culturing, and cryopreserving adherent patient-derived tumor cultures (PDCs). It describes recovering cells from cryopreservation, expanding initial cultures to establish a master seed stock in multiple flasks, and routine sub-culturing. The general workflow and specific steps include thawing cells, culturing in tissue culture flasks, enzymatically dissociating cells, centrifuging, and resuspending cells in fresh media for splitting or
- Author
- Naveen Kumar
- Language
- EN