Opening book details…
Can I read Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies on EtoBox?
Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies by Erika Toneth Ponce Ayala; Fernanda Alves Dias de Sousa; José Dirceu Vollet-Filho; Marlon Rodrigues Garcia; Leonardo de Boni; Vanderlei Salvador Bagnato; Sebastião Pratavieira is a Medicine article available to read on EtoBox.
What is Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies about?
Sono-photodynamic therapy is a promising anticancer technique based on the combination of sonodynamic and photodynamic therapy to improve the cancer treatment effectiveness. This study was aimed at analyzing the effects of the sono-photodynamic (SPD) activity on protoporphyrin IX (PpIX) solution and PpIX-loaded rat liver. In vitro, PpIX 5 μM solutions were irradiated with light (635 nm, 30-50 mW/cm), ultrasound (1 MHz, 1-2 W/cm) and both. The PpIX absorption spectra recorded over exposure time revealed that the PpIX decay rate induced by SPD activity (combined irradiation) was approximately the sum of those induced by photodynamic and sonodynamic activity. In vivo, rats were intraperitoneally injected with 5-aminolevulinic acid at the dose of 500 mg/kg weight. After 3 h of injection, the PpIX-loaded livers were irradiated with light (635 nm, 180 ± 9 J/cm), ultrasound (1.0 MHz, 770 ± 40 J/cm) and both using a single probe capable of illuminating and sonicating the liver simultaneously. After 30 h, the liver damage induced by each protocol was analyzed histologically. It was found that a greater necrosis depth was induced by the SPD activity. These results suggest that the SPD activi
Who reads Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Erika Toneth Ponce Ayala; Fernanda Alves Dias de Sousa; José Dirceu Vollet-Filho; Marlon Rodrigues Garcia; Leonardo de Boni; Vanderlei Salvador Bagnato; Sebastião Pratavieira
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)