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Can I read YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules on EtoBox?
YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules by Konstantin G Chernov; Alain Mechulam; Nadezhda V Popova; David Pastre; Elena S Nadezhdina; Olga V Skabkina; Nina A Shanina; Victor D Vasiliev; Anne Tarrade; Judith Melki; Vandana Joshi; Sonia Baconnais; Flavio Toma; Lev P Ovchinnikov; Patrick A Curmi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules about?
## Background YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. ## Results We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly __in vitro__. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes. ## Conclusion These results suggest that YB-1 may regulate microtubule assembly __in vivo__ and that its interaction with tubulin may contribute to the control of mRNA translation.
Who reads YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Konstantin G Chernov; Alain Mechulam; Nadezhda V Popova; David Pastre; Elena S Nadezhdina; Olga V Skabkina; Nina A Shanina; Victor D Vasiliev; Anne Tarrade; Judith Melki; Vandana Joshi; Sonia Baconnais; Flavio Toma; Lev P Ovchinnikov; Patrick A Curmi
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central; Springer Science and Business Media LLC (ISSN 1471-2091)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)