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Mitochondrial Medicine: Volume 2: Assessing Mitochondria (Methods in Molecular Biology, 2276) by Volkmar Weissig; Marvin Edeas is a nonfiction available to read on EtoBox.

What is Mitochondrial Medicine: Volume 2: Assessing Mitochondria (Methods in Molecular Biology, 2276) about?

Preface Contents Contributors Chapter 1: Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow 1 Introduction 2 Methods to Assess Mitochondrial Dysfunctions 2.1 Basis in Bioenergetic 2.2 Analyses of Maximal ETC Activities 2.3 Structural Analyses: BN-PAGE 2.4 Functional Analyses: Respiration Rates 2.4.1 Devices The O2K Oxygraph The Seahorse XF 2.4.2 Permeabilized Tissues and Cells 2.4.3 Intact Cells 2.5 Mitochondrial Membrane Potential 2.6 Metabolites Dosages and Metabolomics 2.6.1 Metabolite Measurements 2.6.2 Metabolomics 3 Discussion References Chapter 2: Preparation of ``Functional´´ Mitochondria: A Challenging Business 1 Introduction 2 Isolated Mitochondria: Addressing Composition and Function 3 First Step: The Inevitable 4 Isolating ``Intact´´ Mitochondria: A Challenging Business 5 Reproducibility and Quality Control: Guarantee for Successful Analysis 6 Conclusion References Chapter 3: Isolation and Quality Control of Functional Mitochondria 1 Introduction 2 Materials 2.1 Mitochondria Fractionation 2.2 Linear Saccharose Gradient 2.3 Marker Enzyme Assays 2.3.1 Succinate Dehydrogenase (SDH) Assay 2.3.2 Acidic Phosphatase Assay 2.3.3 Ba

Who reads Mitochondrial Medicine: Volume 2: Assessing Mitochondria (Methods in Molecular Biology, 2276)?

Author

Volkmar Weissig; Marvin Edeas

Publisher

Springer US : Imprint: Springer

Published

2021

Language

EN

ISBN

9781071612651

Category

nonfiction

Subjects

Medical, Medicine