Can I read Biotransformation of D‐methionine into L‐methionine in the cascade of four enzymes on EtoBox?

Biotransformation of D‐methionine into L‐methionine in the cascade of four enzymes by Z. Findrik; Đ. Vasić‐Rački is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Biotransformation of D‐methionine into L‐methionine in the cascade of four enzymes about?

## Abstract D‐Methionine was converted to L‐methionine in a reaction system where four enzymes were used. D‐amino acid oxidase (D‐AAO) from __Arthrobacter protophormiae__ was used for the complete conversion of D‐methionine to 2‐oxo‐4‐methylthiobutyric acid. Catalase was added to prevent 2‐oxo‐4‐methylthiobutyric acid decarboxylation. In the second reaction step, L‐phenylalanine dehydrogenase (L‐PheDH) from __Rhodococcus__ sp. was used to convert 2‐ oxo‐4‐methylthiobutyric acid to L‐methionine, and formate dehydrogenase (FDH) from __Candida boidinii__ was added for NADH regeneration. Enzyme kinetics of all enzymes was analyzed in detail. Mathematical models for separate reactions steps, as well as for the complete system were developed and validated in the batch reactor experiments. Complete conversion of D‐methionine to L‐methionine was achieved. Considering that both enzymes act on different substrates, such a system could be easily employed for the synthesis of other amino acids from D‐isomer, as well as from the racemate of a certain amino acid (DL‐amino acid). Biotechnol. Bioeng. 2007;98: 956–967. © 2007 Wiley Periodicals, Inc.

Who reads Biotransformation of D‐methionine into L‐methionine in the cascade of four enzymes?

Author

Z. Findrik; Đ. Vasić‐Rački

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)