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SF-assemblin in Chlamydomonas: Sequence conservation and localization during the cell cycle by Karl-Ferdinand Lechtreck; Carolyn D. Silflow is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is SF-assemblin in Chlamydomonas: Sequence conservation and localization during the cell cycle about?

Previously, SF-assemblin has been identified as the filament-forming component of the striated microtubule-associated fibers (SMAFs), which emerge from the basal bodies in several green flagellates. We have sequenced cDNAs coding for SF-assemblin from Chlamydomonas reinhardtii and C. eugametos. Comparison of the deduced amino acid sequences with the previously described green algal SF-assemblins shows identities between 54 and 71%, indicating a strong drift in sequence. Cells of C. reinhardtii were analyzed by double immunofluorescence using polyclonal anti-SF-assemblin and anti-a-tubulin. In interphase cells, SFassemblin is associated with all four microtubular flagellar roots. During mitosis the SF-assemblin-based cytoskeleton is reorganized; it divides in prophase and is reduced to two dot-like structures at each spindle pole in metaphase. During anaphase, the two dots present at each pole are connected again. In telophase we observed an asymmetrical outgrowth of new fibers. These observations suggest a role for SF-assemblin in reestablishing the microtubular root system characteristic of interphase cells after mitosis.

Who reads SF-assemblin in Chlamydomonas: Sequence conservation and localization during the cell cycle?

Author

Karl-Ferdinand Lechtreck; Carolyn D. Silflow

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0886-1544)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)