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T4 Ligase and Cellulase Production Methods by Gia Hoàng is a document available to read on EtoBox.
What is T4 Ligase and Cellulase Production Methods about?
The document describes the production of T4 ligase and extraction of cellulase. It involves isolating the T4DnL gene from bacteriophage and inserting it into a plasmid to transform E. coli cells. The recombinant cells are cultured and lysed, and the His6-tagged protein is purified using immobilized metal affinity chromatography. It also describes culturing A. niger to extract extracellular cellulase, which is precipitated using ammonium sulfate and further purified using affinity chromatography.
- Author
- Gia Hoàng
- Language
- EN