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Use of pepsin for collagen extraction from the skin of bigeye snapper (Priacanthus tayenus) by Sitthipong Nalinanon; Soottawat Benjakul; Wonnop Visessanguan; Hideki Kishimura is a Agricultural and Biological Sciences article available to read on EtoBox.

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Extraction and some properties of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus) were investigated. Addition of bigeye snapper pepsin (BSP) at a level of 20 kUnits/g of defatted skin resulted in an increased content of collagen extracted from bigeye snapper skin. The yields of collagen from bigeye snapper skin extracted for 48 h with acid and with BSP were 5.31% and 18.74% (dry basis), respectively. With pre-swelling in acid for 24 h, collagen extracted with BSP at a level of 20 kUnits/g of defatted skin for 48 h had a yield of 19.79%, which was greater than that of collagen extracted using porcine pepsin at the same level (13.03%). The skin collagen was characterised to be type I with no disulfide bond. Electrophoretic study revealed slight differences in molecular weight between acid-solubilised collagen and all pepsin-solubilised collagens. The molecular weights of a1 and a2 chains in acid-solubilised collagen were estimated to be 120 and 112 kDa, respectively, whereas a1 and a2 chains of pepsin-solubilised collagens had molecular weights of 118 and 111 kDa, respectively. The result suggested that these pepsin-solubilised collagens might under

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Author

Sitthipong Nalinanon; Soottawat Benjakul; Wonnop Visessanguan; Hideki Kishimura

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)

Published

2007

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)