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The chemistry of single-stranded 4′-DNA radicals: influence of the radical precursor on anaerobic and aerobic strand cleavage by Bernd Giese; Xenia Beyrich-Graf; Peter Erdmann; Mario Petretta; Urs Schwitter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background: Deoxyribosylnucleotide radicals with a radical center at the 4'-position are important intermediates in radical-induced DNA strand cleavage. In the presence of 0,, these DNA radicals yield cleavage products that are partly oxidized. In the past, the postulated peroxide intermediates could not be detected directly because they were unstable under the conditions of either radical generation, the work-up procedure, or the analytical techniques used. We set out to generate and analyze these crucial intermediates in radical-induced DNA strand cleavage under mild conditions. Results: Photolysis experiments with modified singlestranded oligonucleotides generated 4'-DNA radicals that were trapped by 0,. Using MALDI-MS, DNA peroxides could be detected directly. Depending upon the precursor, these peroxides are formed either before or after the cleavage of the single-stranded DNA radical. Reactions in the presence of IsO, and/or HZ'\*0 as well as subsequent transformations to the oxidized cleavage products confirmed the structure of the DNA peroxides. ## Conclusions: Our technique of selective DNA radical generation under mild conditions makes it possible to detect labile reac

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Author

Bernd Giese; Xenia Beyrich-Graf; Peter Erdmann; Mario Petretta; Urs Schwitter

Publisher

Elsevier Science; Elsevier ; Cell Press; Elsevier BV (ISSN 1074-5521)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)