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What is PCR Overview and Basics about?
The polymerase chain reaction (PCR) is a technique used to amplify a single or few copies of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It uses repeated cycles of heating and cooling of the DNA sample to cause DNA replication, using DNA polymerase. During each cycle, the double-stranded DNA template is opened due to heating, primers anneal to the template by cooling, and DNA polymerase synthesizes the complementary strand to form two iden
- Author
- Selva Kumar
- Language
- EN