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A Novel Real-time Ultrasonic Method for Prion Protein Detection Using Plasminogen as a Capture Molecule by Carmen Negredo; Eoin Monks; Torres Sweeney is a scholarly article available to read on EtoBox.

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## Background High resolution ultrasonography (HR-US) can monitor the molecular changes and biochemical interactions between proteins in real-time. The aim of this study was to use HR-US to characterize the real-time interactions between plasminogen coated beads and PrP^Sc^ and to determine if this approach could be applied to the identification of animals affected by prion diseases. Plasminogen, immobilized to beads, was used as a capturing tool for PrP^Sc^ in brain homogenates from scrapie affected sheep and the binding reaction was monitored in real-time in an ultrasonic cell. ## Results Changes in the ultrasonic parameters suggested that three processes occurred during the incubation: binding, protein-protein network formation and precipitation and that these processes occurred in a concentration dependent manner. Conversely, when homogenates from normal sheep were similarly examined, no evidence for the occurrence of these processes was found indicating the specificity of the interaction between the plasminogen coated beads and PrP^Sc^. ## Conclusion These results indicate firstly, that the plasminogen coated beads binded selectively to PrP^Sc^ and secondly, that a HR-US syste

Author

Carmen Negredo; Eoin Monks; Torres Sweeney

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)

Published

2007

Language

EN