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Novel nanocomposite scaffold based on gelatin/PLGA-PEG-PLGA hydrogels embedded with TGF-β1 for chondrogenic differentiation ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Methods 2 2.2.1 Synthesis of PLGA-PEG1500-PLGA triblock copolymer 2 2.2.2 Purification of PLGA-PEG-PLGA nanoparticles 3 2.2.3 Preparation of TGF-β1-Loaded PLGA-PEG-PLGA nanoparticles 3 2.2.4 Preparation of the porous 3-D hydrogel scaffolds 3 2.3 Characterizations of PLGA-PEG-PLGA nanoparticles 3 2.3.1 1H NMR characterization 3 2.3.2 FT-IR characterization 3 2.3.3 Scanning electron microscopy (SEM) analysis of PLGA-PEG-PLGA nanoparticles 3 2.3.4 Morphology analysis of the gelatin/PLGA-PEG-PLGA scaffold 3 2.3.5 Porosity and density properties of the scaffolds 3 2.3.6 In vitro release study 3 2.3.7 In vitro swelling and degradation behavior of the scaffolds 4 2.3.8 Mechanical analysis 4 2.3.9 Cell culture studies 4 2.3.9.1 In vitro cell culture of human dental pulp stem cells (h-DPSCs culture) 4 2.3.9.2 h-DPSCs–seeding On the scaffolds 4 2.3.9.3 SEM of the cell-seeded scaffolds 4 2.3.9.4 In vitro biocompatibility evaluation 4 2.3.9.5 Cell morphology studies 5 2.3.9.6 Histological analysis by Alc
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It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
- Language
- EN
- Category
- nonfiction
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