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Autophagy inhibitor potentiates the antitumor efficacy of apatinib in uterine sarcoma by stimulating PI3K/Akt/mTOR pathway by Shucheng Chen; Lan Yao is a Medicine article available to read on EtoBox.

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## Aim: The present study aims to examine the effects of apatinib combined with autophagy inhibitor 3-methyladenine (3-ma) on the proliferation and apoptosis of human uterine sarcoma in fu-mmt-1 and mes-sa cells and its tumor inhibition effect in xenograft model of uterine sarcoma. ## Methods: Different concentrations of 3-ma and apatinib were used to treat the uterine sarcoma cell lines (mes-sa and fu-mmt-1 cells). the cell viability was detected by cck8 method. flow cytometry was used to detect the apoptosis and cell cycle. wound closure assay and transwell assay were performed to measure the migration ability of cells. western blot was used to determine the apoptosis proteins and autophagy proteins. a nude mice sarcoma xenograft model was established and treated with apatinib alone, 3-ma alone, or combined incubation of them. tumor size of xenograft and the mice survival rate were measured. ## Results: Combination of 3-ma and apatinib significantly inhibited the proliferation and migration ability, but increased the apoptosis rate of uterine sarcoma cells compared to apatinib. the combination of 3-ma and apatinib significantly limited the tumor size of xenograft and increased th

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Author

Shucheng Chen; Lan Yao

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Medicine (Health Sciences)