About this Biochemistry, Genetics and Molecular Biology article

Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro‐computed tomography and vascular replica by Philipp Schneider; Thomas Krucker; Eric Meyer; Alexandra Ulmann‐Schuler; Bruno Weber; Marco Stampanoni; Ralph Müller is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract Recent evidence suggests a close functional relationship between osteogenesis and angiogenesis as well as between bone remodeling and bone vascularization. Consequently, there is a need for visual inspection and quantitative analysis of the bone vasculature. We therefore adapted and implemented two different vascular corrosion casting (VCC) protocols using a polyurethane‐based casting resin in mice for a true three‐dimensional (3D), direct, and simultaneous measurement of bone tissue and vascular morphology by micro‐computed tomography (μCT). For assessment of vascular replicas at the level of capillaries, a vascular contrast perfusion (VCP) protocol was devised using a contrast modality based on a barium sulfate suspension in conjunction with synchrotron radiation (SR) μCT. The vascular morphology quantified using the VCP protocol was compared quantitatively with the results of a previously established method, where the vascular network of cortical bone was derived indirectly from cortical porosity. The presented VCC and VCP protocols have the potential of serving as a valuable method for concomitant 3D quantitative morphometry of the bone tissue and its vasculature. M

Author

Philipp Schneider; Thomas Krucker; Eric Meyer; Alexandra Ulmann‐Schuler; Bruno Weber; Marco Stampanoni; Ralph Müller

Publisher

Wiley

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)