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Proteasome Inhibitor-Induced Apoptosis of Glioma Cells Involves the Processing of Multiple Caspases and Cytochrome c Release by Bettina Wagenknecht; Mirjam Hermisson; Peter Groscurth; Peter Liston; Peter H. Krammer; Michael Weller is a Neuroscience article available to read on EtoBox.
What is Proteasome Inhibitor-Induced Apoptosis of Glioma Cells Involves the Processing of Multiple Caspases and Cytochrome c Release about?
**Abstract:** The proteasome is a multiprotein complex that is involvedin the intracellular protein degradation in eukaryotes. Here, we show thathuman malignant glioma cells are susceptible to apoptotic cell death inducedby the proteasome inhibitors, MG132 and lactacystin. The execution of theapoptotic death program involves the processing of caspases 2, 3, 7, 8, and 9.Apoptosis is inhibited by ectopic expression of X‐linked inhibitor ofapoptosis (XIAP) and by coexposure to the broad‐spectrum caspase inhibitor,benzoyl‐VAD‐fluoromethyl ketone (zVAD‐fmk), but not by the preferentialcaspase 8 inhibitor, crm‐A. It is interesting that specific morphologicalalterations induced by proteasome inhibition, such as dilated roughendoplasmic reticulum and the formation of cytoplasmic vacuoles and densemitochondrial deposits, are unaffected by zVAD‐fmk. Apoptosis is alsoinhibited by ectopic expression of Bcl‐2 or by an inhibitor of proteinsynthesis, cycloheximide. Further, cytochrome __c__ release anddisruption of mitochondrial membrane potential are prominent features ofapoptosis triggered by proteasome inhibition. Bcl‐2 is a stronger inhibitor ofcytochrome __c__ release than zVAD‐fmk. XIAP and
Who reads Proteasome Inhibitor-Induced Apoptosis of Glioma Cells Involves the Processing of Multiple Caspases and Cytochrome c Release?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Bettina Wagenknecht; Mirjam Hermisson; Peter Groscurth; Peter Liston; Peter H. Krammer; Michael Weller
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2000
- Language
- EN
- Field
- Neuroscience (Life Sciences)