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Strategies to evade false positives in the in situ analysis of peptide antibiotics in SDS–PAGE gels by Balaiah Anandaraj; Athinarayanan Selvamanikandan; Prabakar Mittal; Natarajan Elavarasi; Chinnathambi Thangadurai; Vadivel Murugan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Strategies to evade false positives in the in situ analysis of peptide antibiotics in SDS–PAGE gels about?

In situ activity assay is one of the promising techniques for the characterization of peptide antibiotics. This assay was carried out for the peptide purified from a new bacterial isolate Paenibacillus alvei and commercial peptide antibiotic polymixin E. Towards this, the routine and new protocols were tried. Interestingly, the unexpected result of these experiments -the ''switch over activity'' has led us to have further investigations. Here, we have addressed the potential problem in the methodology of in situ assay and demonstrated a fool proof protocol to evade the false positive results.

Who reads Strategies to evade false positives in the in situ analysis of peptide antibiotics in SDS–PAGE gels?

Author

Balaiah Anandaraj; Athinarayanan Selvamanikandan; Prabakar Mittal; Natarajan Elavarasi; Chinnathambi Thangadurai; Vadivel Murugan

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)