Opening book details…
Can I read Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium on EtoBox?
Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium by Ji-Yeon Kang; So-Young Kim; Dooil Kim; Dong-Hyun Kim; Sun-Mi Shin; Sun-Ha Park; Keon-Hee Kim; Heung-Chae Jung; Jae-Gu Pan; Young Hee Joung; Youn-Tae Chi; Ho Zoon Chae; Taeho Ahn; Chul-Ho Yun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium about?
An extreme diversity of substrates and catalytic reactions of cytochrome P450 (P450) enzymes is considered to be the consequence of evolutionary adaptation driven by different metabolic or environmental demands. Here we report the presence of numerous natural variants of P450 BM3 (CYP102A1) within a species of __Bacillus megaterium__. Extensive amino acid substitutions (up to 5% of the total 1049 amino acid residues) were identified from the variants. Phylogenetic analyses suggest that this P450 gene evolve more rapidly than the rRNA gene locus. It was found that key catalytic residues in the substrate channel and active site are retained. Although there were no apparent variations in hydroxylation activity towards myristic acid (C~14~) and palmitic acid (C~16~), the hydroxylation rates of lauric acid (C~12~) by the variants varied in the range of >25-fold. Interestingly, catalytic activities of the variants are promiscuous towards non-natural substrates including human P450 substrates. It can be suggested that CYP102A1 variants can acquire new catalytic activities through site-specific mutations distal to the active site.
Who reads Characterization of diverse natural variants of CYP102A1 found within a species of Bacillus megaterium?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ji-Yeon Kang; So-Young Kim; Dooil Kim; Dong-Hyun Kim; Sun-Mi Shin; Sun-Ha Park; Keon-Hee Kim; Heung-Chae Jung; Jae-Gu Pan; Young Hee Joung; Youn-Tae Chi; Ho Zoon Chae; Taeho Ahn; Chul-Ho Yun
- Publisher
- Springer-Verlag; Springer (Biomed Central Ltd.); Springer Verlag; Heidelberg; Berlin: Springer; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 2191-0855)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)