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Cr3+/Y3+ co-doped persistent luminescence nanoparticles with biological window activation for in vivo repeatable imaging 1 1. Introduction 1 2. Experimental 2 2.1. Synthesis of the ZGSCY phosphors 2 2.2. Synthesis of the ZGSCY NPs 2 2.3. Surface modification of ZGSCY PLNPs 2 2.4. Characterization 2 2.5. Cytotoxicity assay 2 2.6. In vivo persistent luminescence imaging 3 3. Results and discussion 3 3.1. Crystal structure and optical characteristics 3 3.2. Synthesis and characterization of Zn1.3Ga1.4Sn0.3O4:Cr0.005,Y0.003 NPs 3 3.3. Optical properties of the ZGSCY PLNPs 4 3.4. Surface modification of ZGSCY and its cytotoxicity 6 3.5. In vivo PerL imaging, and biodistribution of ZGSCY NPs 9 4. Conclusions 9 Acknowledgements 10 References 10