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Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves by Mónica Santı́n; James M Trout; Lihua Xiao; Ling Zhou; Ellis Greiner; Ronald Fayer is a Immunology and Microbiology article available to read on EtoBox.

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Fifteen dairy farms in seven states on the east coast of the US were each visited on two consecutive years to determinate the prevalence of Cryptosporidium species in pre-weaned (5 days to 2 months) and post-weaned calves (3-11 months), respectively. After each of 971 fecal specimens collected directly from each calf was sieved and subjected to density gradient centrifugation to remove debris and concentrate oocysts, specimens were examined by immunofluorescence microscopy, and polymerase chain reaction (PCR). For all PCR-positive specimens the 18S rRNA gene of Cryptosporidium was sequenced. Cryptosporidium was identified from all farms. Types of housing appeared to have no influence with regard to prevalence of infection. Of 971 calves, 345 were infected with Cryptosporidium (35.5%), but more pre-weaned calves (253 of 503; 50.3%) than post-weaned calves (92 of 468; 19.7%) were found to be infected. A total of 278 PCR-positive specimens characterized by gene sequencing revealed Cryptosporidium parvum, Cryptosporidium andersoni, and two unnamed Cryptosporidium genotypes Bovine B (AY120911) and deer-like genotype (AY120910). The prevalence of these Cryptosporidium species and genotyp

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Author

Mónica Santı́n; James M Trout; Lihua Xiao; Ling Zhou; Ellis Greiner; Ronald Fayer

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)

Published

2004

Language

EN

Field

Immunology and Microbiology (Life Sciences)