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Expression of Heat Shock Protein 27 in Human Renal Cell Carcinoma by Cecilia Sarto; Cristina Valsecchi; Fulvio Magni; Lucia Tremolada; Carmelo Arizzi; Nicoletta Cordani; Stefano Casellato; Giancarlo Doro; Paolo Favini; Roberto A. Perego; Francesca Raimondo; Stefano Ferrero; Paolo Mocarelli; Marzia Galli‐Kienle is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression of Heat Shock Protein 27 in Human Renal Cell Carcinoma about?
Heat shock protein 27 (HSP27, Swiss-Prot accession number P04792) is a component of the large and heterogeneous group of chaperone proteins, and its main functions are inhibition of apoptosis and prevention of aggregation of actin intermediate filament. Modified expression of HSP27 has been described in several cancers including testis, breast, and ovaric cancer. In the present work, 18 renal cell carcinoma (RCC) tissues and homologous normal kidney tissues have been investigated for HSP27 expression by combination of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) separation and Western blotting immunodetection. The results showed significant differences either in expression and in HSP27 isoform numbers in RCC compared to normal kidney. The average number of isoforms was 21 in RCC and 15 in normal tissues with 4.5-5.9 pI range and 18-29 kDa M(r) range. The overexpression was also observed by immunohistochemistry on tissue sections. Only two of RCC samples showed less isoforms than homologous normal samples. Two isoforms were not detected using anti-Ser82 phosphorylated HSP27 antibody, neither in normal nor in RCC samples. Five of all the immunodetected isoforms were
Who reads Expression of Heat Shock Protein 27 in Human Renal Cell Carcinoma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Cecilia Sarto; Cristina Valsecchi; Fulvio Magni; Lucia Tremolada; Carmelo Arizzi; Nicoletta Cordani; Stefano Casellato; Giancarlo Doro; Paolo Favini; Roberto A. Perego; Francesca Raimondo; Stefano Ferrero; Paolo Mocarelli; Marzia Galli‐Kienle
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)