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Broiler Egg Storage Induces Cell Death and Influences Embryo Quality by J.A. Hamidu; Z. Uddin; M. Li; G.M. Fasenko; L.L. Guan; D.R. Barreda is a Agricultural and Biological Sciences article available to read on EtoBox.

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It is well known that egg storage reduces embryo performance, but the fundamental reasons for reduced embryo quality remain unclear. The objective of this study was to investigate possible cellular and molecular mechanisms that might reduce embryo quality after egg storage. Broiler hatching eggs were obtained from the Ross 308 broiler strain, divided into 2 groups, and stored (4 and 14 d) under the same temperature and humidity conditions. Samples of the eggs were used to assess embryo quality by determining daily embryo weight (wet and dry) from 4 to 21 d of incubation. To understand possible cellular and molecular mechanisms that might affect embryo quality, blastoderms (unincubated embryos) were isolated from a sample of eggs from each storage group, dissociated into single cells, and subjected to flow cytometry analysis to differentiate between viable, apoptotic, and necrotic cell populations. Quantitative real-time PCR analysis was used to compare the expression of selected apoptotic genes (Bcl-2 homologous antagonist/killer gene, Bcl-2-associated X gene, Bcl-2-related ovarian killer gene, B-cell lymphoma 2 gene, and B-cell lymphoma xL gene) in blastoderms and embryos (6 d old

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Author

J.A. Hamidu; Z. Uddin; M. Li; G.M. Fasenko; L.L. Guan; D.R. Barreda

Publisher

Poultry Science Association; Oxford University Press; Elsevier BV; Oxford University Press (OUP) (ISSN 0032-5791)

Published

2011

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)