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This document describes the preparation of three biological buffers: citrate buffer with a pH of 5.5, carbonate-bicarbonate buffer with a pH of 10, and glycine-HCl buffer with a pH of 2.5. Standard procedures are provided for making stock solutions of the constituent chemicals and combining them in specific volumes to achieve the target pH values. The pH of each prepared buffer is then measured and recorded using a calibrated pH meter. Preparing buffers of known pH is important for maintaining homeostasis i
- Author
- manojtbgri5793
- Language
- EN