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Tumour suppressor function of MDA-7/IL-24 in human breast cancer by Neill Patani; Anthony Douglas-Jones; Robert Mansel; Wen Jiang; Kefah Mokbel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Introduction Melanoma differentiation associated gene-7 (MDA-7), also known as interleukin (IL)-24, is a tumour suppressor gene associated with differentiation, growth and apoptosis. However, the mechanisms underlying its anti-neoplastic activity, tumour-specificity and efficacy across a spectrum of human cancers have yet to be fully elucidated. In this study, the biological impact of MDA-7 on the behavior of breast cancer (BC) cells is evaluated. Furthermore, mRNA expression of MDA-7 is assessed in a cohort of women with BC and correlated with established pathological parameters and clinical outcome. ## Methods The human BC cell line MDA MB-231 was used to evaluate the in-vitro impact of recombinant human (rh)-MDA-7 on cell growth and motility, using a growth assay, wounding assay and electric cell impedance sensing (ECIS). Localisation of MDA-7 in mammary tissues was assessed with standard immuno-histochemical methodology. BC tissues (n = 127) and normal tissues (n = 33) underwent RNA extraction and reverse transcription, MDA-7 transcript levels were determined using real-time quantitative PCR. Transcript levels were analyzed against tumour size, grade, oestrogen r

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Author

Neill Patani; Anthony Douglas-Jones; Robert Mansel; Wen Jiang; Kefah Mokbel

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1475-2867)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)