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Release kinetics and immunogenicity of parvovirus microencapsulated in PLA/PLGA microspheres by Eszter Pálinkó-Biró; Gábor Rónaszèki; Hans P. Merkle; Bruno Gander is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

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The aim of this work was to examine the immunogenicity of microencapsulated inactivated duck parvovirus in Muscovy duck (Cairina moschata) and goose. Inactivated duck parvovirus suspension was microencapsulated into 14-17 kDa poly(lactide) (PLA) and poly(lactide-co-glycolide) (PLGA50:50H) by coacervation. The in vitro antigen release from individual and mixed PLA and PLGA50:50H microspheres (MS) was biphasic with an initial lag-phase of approx. 10 days followed by a relatively constant release over additional 12 days. By varying the composition of PLA+PLGA50:50H MS mixtures from 3 + 1 to 1+3, the release kinetics could be altered and controlled efficiently. The antigen-loaded MS were injected subcutaneously into ducks. The immune response, expressed as virus neutralisation (VN) titres, after single administration of MS was modest, i.e. below 200 over the 6 weeks tested, unless the animals were pre-immunised 3 weeks before injecting the MS. The weak immune response was attributed to the low dose injected and inappropriate antigen release kinetics. With pre-immunised animals, however, the results were encouraging and showed that the encapsulated parvovirus was immunogenic.

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Author

Eszter Pálinkó-Biró; Gábor Rónaszèki; Hans P. Merkle; Bruno Gander

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

2001

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)