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Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor β1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation by Mi-Hye Lee; Amjad Javed; Hyun-Jung Kim; Hong-In Shin; Soraya Gutierrez; Je-Yong Choi; Vicky Rosen; Janet L. Stein; André J. van Wijnen; Gary S. Stein; Jane B. Lian; Hyun-Mo Ryoo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The bone morphogenetic protein (BMP)-2 is a potent osteoinductive signal, inducing bone formation in vivo and osteoblast differentiation from non-osseous cells in vitro. The runt domain-related protein Cbfa1/PEBP2␣A/ AML-3 is a critical component of bone formation in vivo and transcriptional regulator of osteoblast differentiation. To investigate the relationship between the extracellular BMP-2 signal, Cbfa1, and osteogenesis, we examined expression of Cbfa1 and osteoblastic genes during the BMP-2 induced osteogenic transdifferentiation of the myoblastic cell line C2C12. BMP-2 treatment completely blocked myotube formation and transiently induced expression of Cbfa1 and the bone-related homeodomain protein Msx-2 concomitant with loss of the myoblast phenotype. While induction of collagen type I and alkaline phosphatase (AP) expression coincided with Cbfa1 expression, Cbfa1 mRNA was strikingly downregulated at the onset of expression of osteopontin (OPN) and osteocalcin (OCN) genes, reflecting the mature osteoblast phenotype. TGF-␤1 treatment effectively suppressed myogenesis and induced Cbfa1 expression but was insufficient to support osteoblast differentiation reflected by the abs

Who reads Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor β1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation?

Author

Mi-Hye Lee; Amjad Javed; Hyun-Jung Kim; Hong-In Shin; Soraya Gutierrez; Je-Yong Choi; Vicky Rosen; Janet L. Stein; André J. van Wijnen; Gary S. Stein; Jane B. Lian; Hyun-Mo Ryoo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)