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Interaction of Sugars, Polysaccharides and Cells with Boronate-containing Copolymers: from Solution to Polymer Brushes by Alexander E. Ivanov; Igor Yu. Galaev; Bo Mattiasson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Interaction of Sugars, Polysaccharides and Cells with Boronate-containing Copolymers: from Solution to Polymer Brushes about?
## Abstract Interaction of mono‐ and disaccharides, polysaccharide particles and yeast cells with boronate‐containing copolymers (BCC) of N‐acryloyl‐__m__‐aminophenylboronic acid (NAAPBA) with __N,N__‐dimethylacrylamide (DMAA) or N‐isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (ΔT~P~), which provided a quantitative measure for the complex formation. Among the sugars typical of non‐reducing ends of glycoproteins the ΔT~P~ decreased in the order: N‐acetylneuraminic acid > xylose ≈ galactose > mannose ≈ fucose ≫ N‐acetylglucosamine. Strong specific adsorption of the BCC on the cross‐linked agarose gel Sepharose CL‐6B (15–30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate‐sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA‐NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous bu
Who reads Interaction of Sugars, Polysaccharides and Cells with Boronate-containing Copolymers: from Solution to Polymer Brushes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Alexander E. Ivanov; Igor Yu. Galaev; Bo Mattiasson
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3499)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)