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Characterization and Bioactivity of Extracellular Vesicles Isolated from Pomegranate by Christian M. Sánchez-López; Mari Cruz Manzaneque-López; Pedro Pérez-Bermúdez; Carla Soler; Antonio Marcilla is a scholarly article available to read on EtoBox.

What is Characterization and Bioactivity of Extracellular Vesicles Isolated from Pomegranate about?

In the current study, extracellular vesicles from pomegranate juice (PgEVs) were isolated for the first time using size exclusion chromatography (SEC). This method permitted us to obtain highly enriched EV samples without most of the non-EV co-isolated proteins. The characterization of PgEVs through nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) allowed the determination of vesicles' concentration/volume, size, and morphology. It was confirmed from the analytical data that PgEVs contain a homogeneous population of vesicles, with a dimension and structure comparable to plant-derived EVs. Proteomic analyses by LC-MS/MS led to the characterization of 131 proteins, and several of them were related commonly to the biogenesis and transport of EVs, and/or proposed as EV markers. PgEVs exerted anti-inflammatory, antioxidant and wound-healing effects when added to the cultures of monocytic (THP-1) and intestinal (Caco-2) cell lines, respectively.

Author

Christian M. Sánchez-López; Mari Cruz Manzaneque-López; Pedro Pérez-Bermúdez; Carla Soler; Antonio Marcilla

Published

2022

Language

EN