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Fluoroquinolone resistance and mutational profile of gyrA in pulmonary MDR tuberculosis patients by Kabir, Saba; Tahir, Zarfishan; Mukhtar, Nadia; Sohail, Muhammad; Saqalein, Muhammad; Rehman, Abdul is a Medicine article available to read on EtoBox.

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## Background Fluoroquinolones (FQs) are potential drugs that inhibit DNA synthesis and are used in the treatment of multidrug-resistant tuberculosis (TB) and short-term anti-TB regimens. In recent years, a high proportion of FQ resistance has been observed in __Mycobacterium tuberculosis__ isolates. The development of FQ resistance in multidrug-resistant TB negatively impacts patient treatment outcome and is a serious threat to control of TB. ## Methods The study included a total of 562 samples from patients with pulmonary TB that had been on anti-tuberculosis therapy. MTBDR__sl__ assays were performed for the molecular detection of mutations. Sequence analysis was performed for the characterization and mutational profiling of FQ-resistant isolates. ## Results FQ resistance was observed in 104 samples (18.5%), most of which were previously treated and treatment failure cases. A total of 102 isolates had mutations in DNA gyrase subunit A (__gyrA__), while mutations in __gyrB__ were observed in only two isolates. Mutational analysis revealed that the mutations mostly alter codons 94 (replacing aspartic acid with glycine, D94G) and 90 (replacing alanine with valine, A90V). In MDR and

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Author

Kabir, Saba; Tahir, Zarfishan; Mukhtar, Nadia; Sohail, Muhammad; Saqalein, Muhammad; Rehman, Abdul

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2466)

Published

2020

Language

EN

Field

Medicine (Health Sciences)