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A bacterial vaccine polypeptide protective against nontypable Haemophilus influenzae by Whitby, Paul W.; Morton, Daniel J.; Mussa, Huda J.; Mirea, Lucia; Stull, Terrence L. is a Medicine article available to read on EtoBox.

What is A bacterial vaccine polypeptide protective against nontypable Haemophilus influenzae about?

Nontypeable strains of Haemophilus influenzae (NTHi) are one of the most common cause of otitis media and the most frequent infection associated with exacerbations of chronic obstructive pulmonary disease; there is currently no vaccine in the U.S. to prevent NTHi. Using bioinformatics and structural vaccinology, we previously identified several NTHi species-conserved and sequence-conserved peptides that mediate passive protection in the rat model of infection. Using these, and similar peptides, we designed Hi Poly 1, a Bacterial Vaccine Polypeptide, comprising 9 unique peptides from 6 different surface proteins. Recombinant Hi Poly 1 was purified by affinity chromatography. Forty chinchillas were immunized three times with 200 μg of Hi Poly 1 with alum adjuvant; similarly, 41 controls were immunized with adjuvant alone. The average Log IgG titer among immunized animals was 17.04, and IgG antibodies against each component peptide were detected. In the infant rat model, antisera from immunized chinchillas provided significant passive protection compared to PBS (p = 0.01) and pre-immune sera (p = 0.03). In the established chinchilla model of NTHi otitis media, the vaccinated group cle

Who reads A bacterial vaccine polypeptide protective against nontypable Haemophilus influenzae?

Author

Whitby, Paul W.; Morton, Daniel J.; Mussa, Huda J.; Mirea, Lucia; Stull, Terrence L.

Publisher

Elsevier Science; Elsevier ; Elsevier BV; ScienceOpen (ISSN 0264-410X)

Published

2020

Language

EN

Field

Medicine (Health Sciences)