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Automated ‘X-Y’ robot for peptide synthesis with microwave heating: application to difficult peptide sequences and protein domains by Leila Malik; A. Pernille Tofteng; Søren L. Pedersen; Kasper K. Sørensen; Knud J. Jensen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Precise microwave heating has emerged as a valuable method to aid solid‐phase peptide synthesis (SPPS). New methods and reliable protocols, as well as their embodiment in automated instruments, are required to fully use this potential. Here we describe a new automated robotic instrument for SPPS with microwave heating, report protocols for its reliable use and report the application to the synthesis of long sequences, including the β‐amyloid 1‐42 peptide. The instrument is built around a valve‐free robot originally developed for parallel peptide synthesis, where the robotic arm transports reagents instead of pumping reagents via valves. This is the first example of an ‘X‐Y’ robotic microwave‐assisted synthesizer developed for the assembly of long peptides. Although the instrument maintains its capability for parallel synthesis at room temperature, in this paper, we focus on sequential peptide synthesis with microwave heating. With this valve‐free instrument and the protocols developed for its use, fast and efficient syntheses of long and difficult peptide sequences were achieved. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.

Who reads Automated ‘X-Y’ robot for peptide synthesis with microwave heating: application to difficult peptide sequences and protein domains?

Author

Leila Malik; A. Pernille Tofteng; Søren L. Pedersen; Kasper K. Sørensen; Knud J. Jensen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-2617)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)