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Can I read The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines on EtoBox?
The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines by Adriana Miti Nakahata; Barbara Mayer; Christian Ries; Cláudia Alessandra Andrade de Paula; Marisa Karow; Peter Neth; Misako U. Sampaio; Marianne Jochum; Maria Luiza V. Oliva is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines about?
## Abstract Supplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from__Enterolobium contortisiliquum__, it also blocks human neutrophil elastase (__K__~iapp~=4.3 nm) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogen-induced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0–2.5 μmrather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMP activation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination.
Who reads The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Adriana Miti Nakahata; Barbara Mayer; Christian Ries; Cláudia Alessandra Andrade de Paula; Marisa Karow; Peter Neth; Misako U. Sampaio; Marianne Jochum; Maria Luiza V. Oliva
- Publisher
- Walter de Gruyter GmbH
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)