Can I read Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes on EtoBox?

Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes by Chen, Han; SchÃ1⁄4rch, Christian M.; Noble, Kevin; Kim, Kenneth; Krutzik, Peter O.; OâDonnell, Erika; Vander Tuig, Jason; Nolan, Garry P.; McIlwain, David R. is a Immunology and Microbiology article available to read on EtoBox.

What is Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes about?

## Background Cryopreserved human peripheral blood mononuclear cells (PBMCs) are a commonly used sample type for a variety of immunological assays. Many factors can affect the quality of PBMCs, and careful consideration and validation of an appropriate PBMC isolation and cryopreservation method is important for well-designed clinical studies. A major point of divergence in PBMC isolation protocols is the collection of blood, either directly into vacutainers pre-filled with density gradient medium or the use of conical tubes containing a porous barrier to separate the density gradient medium from blood. To address potential differences in sample outcome, we isolated, cryopreserved, and compared PBMCs using parallel protocols differing only in the use of one of two common tube types for isolation. ## Methods Whole blood was processed in parallel using both Cell Preparation TubesTM (CPT, BD Biosciences) and LymphoprepTM Tubes (Axis-Shield) and assessed for yield and viability prior to cryopreservation. After thawing, samples were further examined by flow cytometry for cell yield, cell viability, frequency of 10 cell subsets, and capacity for stimulation-dependent CD4+ and CD8+ T cell

Who reads Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes?

Author

Chen, Han; SchÃ1⁄4rch, Christian M.; Noble, Kevin; Kim, Kenneth; Krutzik, Peter O.; OâDonnell, Erika; Vander Tuig, Jason; Nolan, Garry P.; McIlwain, David R.

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2000-; Springer Science and Business Media LLC (ISSN 1471-2172)

Published

2020

Language

EN

Field

Immunology and Microbiology (Life Sciences)