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Neochamaejasmin A Induces Mitochondrial-Mediated Apoptosis in Human Hepatoma Cells via ROS-Dependent Activation of the ERK1/2/JNK Signaling Pathway 1 1. Introduction 1 2. Materials and Methods 2 2.1. Materials 2 2.2. Cell Culture 2 2.3. Cell Viability Assay 2 2.4. Morphological Assay 2 2.5. Reactive Oxygen Analysis 2 2.6. Detection of Intracellular Oxidoreductase SOD and CAT 2 2.7. Cell Apoptosis Analysis 3 2.8. Mitochondrial Membrane Potential Analysis 3 2.9. Western Blot Analysis 4 2.10. Statistical Analysis 4 3. Results 5 3.1. NCA Inhibits HepG2 Cell Proliferation and Induces Cell Morphology Changes 5 3.2. NCA Induces HepG2 Cell Apoptosis and Regulates the Levels of Apoptosis-Related Proteins 5 3.3. NCA Induces a Mitochondrial-Dependent Apoptotic Pathway in HepG2 Cells 5 3.4. NCA Induces the Production of Reactive Oxygen Species (ROS) and Regulates the Activities of Antioxidant Enzymes 5 3.5. Effect of NCA on the ERK1/2/JNK Signaling Pathway in HepG2 Cells 5 3.6. The ERK1/2/JNK Signaling Pathway Is Involved in NCA-Induced HepG2 Cell Apoptosis 6 3.7. NCA Induces Cell Apoptosis and Increases ROS Level in BEL-7402 Cells 6 4. Discussion 7 5. Conclusions 11 Data Availability 11 Confl

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Aspose Ltd.

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EN