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Ptimization of Co-immobilization of Cellulase and Î2-glucosidase by Wu, D Y; Zhuang, M Z; Su, G X; Gao, P F; Jin, Y Z; Fu, H Y is a Agricultural and Biological Sciences article available to read on EtoBox.

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Cellulase can hydrolyze cellulose to produce reducing sugars such as cellobiose and cellotriose. β-glucosidas can further hydrolyze cellobiose and cellobiose produced by cellulase to produce glucose. According to the hydrolysis mechanisms of cellulase and β-glucosidas, we selected two enzyems, cellulase and β-glucosidase to study optimization of co-immobilization of cellulase and β-glucosidas. Meanwhile, we selected two materials, activated carbon and sodium alginate to co-immobilize cellulase and β-glucosidas by the immobilization method of embedding-adsorption. The immobilization conditions, alginate, CaCl 2 and activated carbon was optimized. The results showed that the optimum concentrations of sodium alginate and CaCl 2 were 2% and 2%, respectively, and the quality of activated carbon is 0.15 g. The optimal ratio of cellulase to β -glucosidase was 1:1.5. The results indicated that cellulase and β-glucosidase had a synergistic effect and that their compound degradation of cellulose was better than the separate effects of the two enzymes acting independently.

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Author

Wu, D Y; Zhuang, M Z; Su, G X; Gao, P F; Jin, Y Z; Fu, H Y

Publisher

IOP Publishing (ISSN 1755-1307)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Physical Sciences)