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Cell-Free Urine and Plasma DNA Mutational Analysis Predicts Neoadjuvant Chemotherapy Response and Outcome in Patients with Muscle-Invasive Bladder Cancer by Emil Christensen; Iver Nordentoft; Karin Birkenkamp-Demtröder; Sara K. Elbæk; Sia V. Lindskrog; Ann Taber; Tine G. Andreasen; Trine Strandgaard; Michael Knudsen; Philippe Lamy; Mads Agerbæk; Jørgen B. Jensen; Lars Dyrskjøt is a scholarly article available to read on EtoBox.

What is Cell-Free Urine and Plasma DNA Mutational Analysis Predicts Neoadjuvant Chemotherapy Response and Outcome in Patients with Muscle-Invasive Bladder Cancer about?

## Abstract ## Purpose: To investigate the use of plasma and urine DNA mutation analysis for predicting neoadjuvant chemotherapy (NAC) response and oncological outcome in patients with muscle-invasive bladder cancer. ## Experimental Design: Whole-exome sequencing of tumor and germline DNA was performed for 92 patients treated with NAC followed by radical cystectomy (RC). A custom NGS-panel capturing approximately 50 mutations per patient was designed and used to track mutated tumor DNA in plasma and urine. A total of 447 plasma samples, 281 urine supernatants, and 123 urine pellets collected before, during, and after treatment were analyzed. Patients were enrolled from 2013 to 2019, with a median follow-up time of 41.3 months after RC. ## Results: We identified tumor DNA before NAC in 89% of urine supernatants, 85% of urine pellets, and 43% of plasma samples. Tumor DNA levels were higher in urine supernatants and urine pellets compared with plasma samples (__P__ < 0.001). In plasma, detection of circulating tumor DNA (ctDNA) before NAC was associated with a lower NAC response rate (__P__ < 0.001). Detection of tumor DNA after NAC was associated with lower response rates in plasma,

Author

Emil Christensen; Iver Nordentoft; Karin Birkenkamp-Demtröder; Sara K. Elbæk; Sia V. Lindskrog; Ann Taber; Tine G. Andreasen; Trine Strandgaard; Michael Knudsen; Philippe Lamy; Mads Agerbæk; Jørgen B. Jensen; Lars Dyrskjøt

Publisher

American Association for Cancer Research (AACR)

Published

2023

Language

EN