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The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10 by Yang, Xue; Li, Siting; Wu, Ying; Ge, Feng; Chen, Ying; Xiong, Qian is a Medicine article available to read on EtoBox.

What is The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10 about?

## Background Circular RNAs (CircRNAs) are biologically active RNAs. CDR1as is one such circRNA previously reported to be a microRNA-7 (miR-7) sponge, thereby regulating associated gene expression. The specific underlying molecular mechanisms of CDR1as biology, however, remain largely unknown. ## Methods We performed CDR1as knockdown in order to explore its function in cell proliferation, migration, the cell cycle, and tumorigenesis. We further employed quantitative proteomic analyses and associated bioinformatics strategies to globally assess CDR1as-regulated proteins (CRPs). Western blotting and immunofluorescence staining were used to validate the proteomic results. We additionally investigated a specific link between TMED2, TMED10, and miR-7 via a dual-luciferase reporter system, and generated CDR1as knockout cell lines via CRISPR/Cas9 editing. ## Results We identified 353 proteins dysregulated upon CDR1as knockdown in 293 T cells. These CRPs were found to interact with one another and to play key roles in certain cellular pathways. Two such proteins, TMED2 and TMED10, were found to specifically contribute to the influence of CDR1as on cell proliferation. CDR1as may regulate th

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Author

Yang, Xue; Li, Siting; Wu, Ying; Ge, Feng; Chen, Ying; Xiong, Qian

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2001]-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2407)

Published

2020

Language

EN

Field

Medicine (Health Sciences)