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Can I read Macrophage Migration Inhibitory Factor (MIF) Enzymatic Activity and Lung Cancer on EtoBox?
Macrophage Migration Inhibitory Factor (MIF) Enzymatic Activity and Lung Cancer by Leona Mawhinney; Michelle E. Armstrong; Ciaran O’Reilly; Richard Bucala; Lin Leng; Gunter Fingerle-Rowson; Darren Fayne; Michael P. Keane; Aisling Tynan; Lewena Maher; Gordon Cooke; David Lloyd; Helen Conroy; Seamas C. Donnelly is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Macrophage Migration Inhibitory Factor (MIF) Enzymatic Activity and Lung Cancer about?
The cytokine macrophage migration inhibitory factor (MIF) possesses unique tautomerase enzymatic activity, which contributes to the biological functional activity of MIF. In this study, we investigated the effects of blocking the hydrophobic active site of the tautomerase activity of MIF in the pathogenesis of lung cancer. To address this, we initially established a Lewis lung carcinoma (LLC) murine model in Mif-KO and wild-type (WT) mice and compared tumor growth in a knock-in mouse model expressing a mutant MIF lacking enzymatic activity (Mif (P1G)). Primary tumor growth was significantly attenuated in both Mif-KO and Mif (P1G) mice compared with WT mice. We subsequently undertook a structure-based, virtual screen to identify putative small molecular weight inhibitors specific for the tautomerase enzymatic active site of MIF. From primary and secondary screens, the inhibitor SCD-19 was identified, which significantly attenuated the tautomerase enzymatic activity of MIF in vitro and in biological functional screens. In the LLC murine model, SCD-19, given intraperitoneally at the time of tumor inoculation, was found to significantly reduce primary tumor volume by 90% (p < 0.001) co
Who reads Macrophage Migration Inhibitory Factor (MIF) Enzymatic Activity and Lung Cancer?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Leona Mawhinney; Michelle E. Armstrong; Ciaran O’Reilly; Richard Bucala; Lin Leng; Gunter Fingerle-Rowson; Darren Fayne; Michael P. Keane; Aisling Tynan; Lewena Maher; Gordon Cooke; David Lloyd; Helen Conroy; Seamas C. Donnelly
- Publisher
- The Feinstein Institute for Medical Research; Springer (Biomed Central Ltd.); North Shore - Long Jewish Research Institute; Baltimore Md.: Johns Hopkins University Press; Manhasset NY: North Shore-Long Island Jewish Research Institute; Manhasset NY: Feinstein Institute for Medical Research; [London]: BioMed Central; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Test accounts (ISSN 1076-1551)
- Published
- 2015
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)