About this Engineering article
Biosensing of benzene derivatives in the environment by luminescent Escherichia coli by Eiry Kobatake; Tomohisa Niimi; Tetsuya Haruyama; Yoshihito Ikariyama; Masuo Aizawa is a Engineering article available to read on EtoBox.
Sensitive and convenient biosensing of environmental pollutants has been developed by fusing a gene of firefly luciferase to the TOL plasmid. TOL plasmid of Pseudomonas putida encodes a series of enzymes for degradation of benzene and its derivatives. The expression of these enzymes is controlled with the regulating proteins xylR and xylS, whose promoters are activated in the presence of aromatic compounds. The structural gene of firefly luciferase, as a reporter enzyme, was inserted under the control of the promoter of xylS protein, and gene fusion plasmid pTSN316 was constructed. The recombinant Escherichia coli transformed with this plasmid was applied to the environmental biosensing of benzene derivatives. The expression of luciferase was induced in the presence of aromatic compounds and the lower detection limit for m-xylene was 5/xM.
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Eiry Kobatake; Tomohisa Niimi; Tetsuya Haruyama; Yoshihito Ikariyama; Masuo Aizawa
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 1995
- Language
- EN
- Field
- Engineering (Physical Sciences)