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Smartphone-based 3D-printed electrochemiluminescence enzyme biosensor for reagentless glucose quantification in real matrices by Donato Calabria; Elisa Lazzarini; Andrea Pace; Ilaria Trozzi; Martina Zangheri; Stefano Cinti; Marinella Difonzo; Giovanni Valenti; Massimo Guardigli; Francesco Paolucci; Mara Mirasoli is a scholarly article available to read on EtoBox.

What is Smartphone-based 3D-printed electrochemiluminescence enzyme biosensor for reagentless glucose quantification in real matrices about?

Three-dimensional (3D) printed electrochemical devices are increasingly used in point-of-need and point-of-care testing. They show several advantages such as simple fabrication, low cost, fast response, and excellent selectivity and sensitivity in small sample volumes. However, there are only a few examples of analytical devices combining 3D-printed electrodes with electrochemiluminescence (ECL) detection, an electrochemical detection principle widely employed in clinical chemistry analysis. Herein, a portable, 3D-printed miniaturized ECL biosensor for glucose detection has been developed, based on the luminol/H2O2 ECL system and employing a two-electrode configuration with carbon black-doped polylactic acid (PLA) electrodes. The ECL emission is obtained by means of a 1.5V AA alkaline battery and detected using a smartphone camera, thus providing easy portability of the analytical platform. The ECL system was successfully applied for sensing H2O2 and, upon coupling the luminol/H2O2 system with the enzyme glucose oxidase, for glucose detection. The incorporation of luminol and glucose oxidase in an agarose hydrogel matrix allowed to produce ECL devices preloaded with the reagents re

Author

Donato Calabria; Elisa Lazzarini; Andrea Pace; Ilaria Trozzi; Martina Zangheri; Stefano Cinti; Marinella Difonzo; Giovanni Valenti; Massimo Guardigli; Francesco Paolucci; Mara Mirasoli

Publisher

Elsevier BV

Published

2023

Language

EN