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Understanding protein kinase CK2 mis-regulation upon F508del CFTR expression by Andrea Venerando; Mario A. Pagano; Kendra Tosoni; Flavio Meggio; Diane Cassidy; Michelle Stobbart; Lorenzo A. Pinna; Anil Mehta is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Understanding protein kinase CK2 mis-regulation upon F508del CFTR expression about?

We review areas of overlap between nucleoside diphosphate kinase (NDPK; nm23) and two proteins manifesting an equivalent diversity of action, each with many thousands of publications. The first is a constitutively active protein kinase, CK2 (formerly casein kinase 2), that includes NDPK amongst its hundreds of targets. The second is an enigmatic member of the ATP-binding cassette (ABC) family of membrane pumps that normally hydrolyse ATP to transport substrates. Yet our unusual family member (ABCC7) is not a pump but, uniquely, acts as a regulated anion channel. ABCC7 is the cystic fibrosis transmembrane conductance regulator (CFTR), and we discuss the highly prevalent CFTR mutation (F508del CFTR) in terms of the uncertainties surrounding the molecular basis of cystic fibrosis that cloud approaches to corrective therapy. Using lysates from cells stably expressing either wild-type or F508del CFTR, incubated with the CK2 substrate GTP, we show that the phosphoproteome of F508del CFTR-expressing cells both differs from wild-type CFTR-expressing cells and is significantly enhanced in intensity by ∼1.5-fold (__p__ < 0.05, paired __t__ test with Bonferroni correction, __n__ = 4). Phospho

Who reads Understanding protein kinase CK2 mis-regulation upon F508del CFTR expression?

Author

Andrea Venerando; Mario A. Pagano; Kendra Tosoni; Flavio Meggio; Diane Cassidy; Michelle Stobbart; Lorenzo A. Pinna; Anil Mehta

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0028-1298)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)