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Antigenic expression of human metastatic prostate cancer cell lines for in vitro multiple-targeted α-therapy with 213Bi-conjugates by Yong Li; Syed M. Abbas Rizvi; Julie M. Blair (nee Brown); Paul J. Cozzi; Chang F. Qu; Kim T. Ow; Patrick N. Tam; Alan C. Perkins; Pamela J. Russell; Barry J. Allen is a Medicine article available to read on EtoBox.

What is Antigenic expression of human metastatic prostate cancer cell lines for in vitro multiple-targeted α-therapy with 213Bi-conjugates about?

## Purpose: Control of metastatic prostate cancer (cap) is an elusive objective. some 30% of patients with clinically localized cap will develop micrometastatic disease. defining the expression of tumor-associated antigens on cap will enable appropriate selection of therapeutic targets. ## Methods and materials: The expression of tumor-associated antigens on cap cell lines (pc-3, du 145, and lncap-ln3) was detected by immunohistochemistry and flow cytometry. test and control alpha-conjugates were prepared using monoclonal antibodies, an inhibitor, plasminogen activator inhibitor type 2, that binds to the cell-membrane-bound protease, urokinase plasminogen activator, and a control protein labeled with (213)bi using standard methods. these were used singly or together against three different cap cell lines in vitro. the cytotoxicity of the alpha-conjugates was assessed using the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2h-tetrazolium, inner salt] (mts) assay. ## Results: The pc-3 and du 145 cancer cell lines expressed antigens that bind monoclonal antibodies blca-38 and #394 (mouse anti-human urokinase plasminogen activator b-chain) but not j591. the

Who reads Antigenic expression of human metastatic prostate cancer cell lines for in vitro multiple-targeted α-therapy with 213Bi-conjugates?

Author

Yong Li; Syed M. Abbas Rizvi; Julie M. Blair (nee Brown); Paul J. Cozzi; Chang F. Qu; Kim T. Ow; Patrick N. Tam; Alan C. Perkins; Pamela J. Russell; Barry J. Allen

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)

Published

2004

Language

EN

Field

Medicine (Health Sciences)