Can I read Detection of the R553X DNA single point mutation related to cystic fibrosis by a “chiral box”D-lysine-peptide nucleic acid probe by capillary electrophoresis on EtoBox?

Detection of the R553X DNA single point mutation related to cystic fibrosis by a “chiral box”D-lysine-peptide nucleic acid probe by capillary electrophoresis by Tullia Tedeschi; Marcella Chiari; Gianni Galaverna; Stefano Sforza; Marina Cretich; Roberto Corradini; Rosangela Marchelli is a Engineering article available to read on EtoBox.

What is Detection of the R553X DNA single point mutation related to cystic fibrosis by a “chiral box”D-lysine-peptide nucleic acid probe by capillary electrophoresis about?

In order to recognize the presence of the R553X point mutation of the cystic fibrosis (CF) gene in the human genome, a peptide nucleic acid (PNA) complementary to the mutated gene tract and bearing three adjacent chiral monomers based on D-lysine (chiral box) was synthesized and used as a probe in CE. Binding specificity was preliminarily studied with complementary and mismatched oligonucleotides by UV spectroscopy, electrospray MS, and electrophoresis, indicating a very high sequence selectivity. The chiral PNA probe was then hybridized to cyanine-5-labeled DNA samples (186 bp), obtained by PCR amplification, respectively, from: (a) normal homozygous subjects (wtDNA), (b) CF-affected homozygous subjects (mutDNA), (c) heterozygous subjects (healthy carriers) and denatured at low ionic strength. The PNA-DNA mixture was directly analyzed by CE with LIF detection: a new signal corresponding to the PNA-mutDNA duplex was observed, in the case of CF-affected homozygous subjects, whereas for the sample containing the mismatched sequence (normal homozygous wtDNA) only the signal corresponding to ssDNA (ss, single strand) was detected. In the case of heterozygous DNA, both PNA-mutDNA duplex

Who reads Detection of the R553X DNA single point mutation related to cystic fibrosis by a “chiral box”D-lysine-peptide nucleic acid probe by capillary electrophoresis?

Author

Tullia Tedeschi; Marcella Chiari; Gianni Galaverna; Stefano Sforza; Marina Cretich; Roberto Corradini; Rosangela Marchelli

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2005

Language

EN

Field

Engineering (Physical Sciences)