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Identification of Target Cells of Human Papillomavirus 18 Using Squamocolumnar Junction Organoids by Yusuke Toyohara; Ayumi Taguchi; Yoshiyuki Ishii; Daisuke Yoshimoto; Miki Yamazaki; Hiroko Matsunaga; Kazuma Nakatani; Daisuke Hoshi; Saki Tsuchimochi; Misako Kusakabe; Satoshi Baba; Akira Kawata; Masako Ikemura; Michihiro Tanikawa; Kenbun Sone; Mayuyo Uchino‐Mori; Tetsuo Ushiku; Haruko Takeyama; Katsutoshi Oda; Kei Kawana; Yoshitaka Hippo; Yutaka Osuga is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification of Target Cells of Human Papillomavirus 18 Using Squamocolumnar Junction Organoids about?

## Abstract Human papillomavirus 18 (HPV18) is a highly malignant HPV genotype among high‐risk HPVs, characterized by the difficulty of detecting it in precancerous lesions and its high prevalence in adenocarcinomas. The cellular targets and molecular mechanisms underlying its infection remain unclear. In this study, we aimed to identify the cells targeted by HPV18 and elucidate the molecular mechanisms underlying HPV18 replication. Initially, we established a lentiviral vector (HPV18LCR‐GFP vector) containing the HPV18 long control region promoter located upstream of __EGFP__. Subsequently, HPV18LCR‐GFP vectors were transduced into patient‐derived squamocolumnar junction organoids, and the presence of GFP‐positive cells was evaluated. Single‐cell RNA sequencing of GFP‐positive and GFP‐negative cells was conducted. Differentially expressed gene analysis revealed that 169 and 484 genes were significantly upregulated in GFP‐positive and GFP‐negative cells, respectively. Pathway analysis showed that pathways associated with cell cycle and viral carcinogenesis were upregulated in GFP‐positive cells, whereas keratinization and mitophagy/autophagy‐related pathways were upregulated in GFP

Who reads Identification of Target Cells of Human Papillomavirus 18 Using Squamocolumnar Junction Organoids?

Author

Yusuke Toyohara; Ayumi Taguchi; Yoshiyuki Ishii; Daisuke Yoshimoto; Miki Yamazaki; Hiroko Matsunaga; Kazuma Nakatani; Daisuke Hoshi; Saki Tsuchimochi; Misako Kusakabe; Satoshi Baba; Akira Kawata; Masako Ikemura; Michihiro Tanikawa; Kenbun Sone; Mayuyo Uchino‐Mori; Tetsuo Ushiku; Haruko Takeyama; Katsutoshi Oda; Kei Kawana; Yoshitaka Hippo; Yutaka Osuga

Publisher

John Wiley and Sons Inc.

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)