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Strong Ion-exchange Centrifugal Partition Chromatography as an Efficient Method for the Large-scale Purification of Glucosinolates by Alix Toribio; Jean-Marc Nuzillard; Jean-Hugues Renault is a Chemistry article available to read on EtoBox.

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The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIX-CPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6 g of glucoraphanin were obtained in one step in 2.5 and 3.5 h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume).

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Author

Alix Toribio; Jean-Marc Nuzillard; Jean-Hugues Renault

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)